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MB Sample ID: SA165094
| Local Sample ID: | LP27_pre_CSF_M |
| Subject ID: | SU001857 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 25(IQR 22-31) |
| Gender: | Male and female |
| Human Race: | European |
| Human Medications: | None |
| Human Prescription Otc: | None |
| Human Smoking Status: | None |
| Human Alcohol Drug Use: | None |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002890 | AN002891 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | Reversed phase |
| Chromatography system | Shimadzu 20AD | Shimadzu 20AD |
| Column | Shodex NH2P-40 2E (250 x 2mm,4um) | Raptor Biphenyl (150 × 2.1 mm,2.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | UNSPECIFIED | UNSPECIFIED |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH002142 |
| Chromatography Summary: | Ten µL of the extract was injected into the column through a CTC PAL autosampler. The samples were separated in HILIC mode using a polymer-based NH2 column (250 × 2 mm, 4 µm) (Asahipak NH2P-40 2E, Shodex, Japan). The optimal LC conditions were as follows: Mobile phase A: 95% H2O with 20 mM (NH4)2CO3 and 5% ACN, pH 9.8. Mobile phase B: 100% ACN. The gradient was: 0 - 3.5 min 95% B, 3.6 - 8 min 85% B, 8.1 - 13 min 75% B, 14 - 30 min 0% B, 31 - 41 min 95% B, 41.1 min stop. The flow rate was 200 µL/min, and the column temperature was held at 25 ºC. |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Shodex NH2P-40 2E (250 x 2mm,4um) |
| Column Pressure: | 900 psi - 2400 psi |
| Column Temperature: | 25 |
| Flow Gradient: | Constant |
| Flow Rate: | 200 µL/min |
| Injection Temperature: | 4 |
| Sample Injection: | 10 uL |
| Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium acetate, pH 9.8 |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 45 min |
| Oven Temperature: | 25 |
| Sample Loop Size: | 10 |
| Sample Syringe Size: | 100 |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH002143 |
| Chromatography Summary: | Ten µL of the extract was injected into the column through a CTC PAL autosampler. The samples were separated in the RP model using a Raptor Biphenyl column (150 × 2.1 mm, 2.7 µm) (Restek, PA). The LC conditions were as follows: Mobile phase A: 90% H2O with 0.1% formic acid and 10% MEOH, pH 4.0. Mobile phase B: MEOH-IPA (50:50, v/v) with 0.1% formic acid. The gradient was: 0 - 2 min 10% B, 2.1 - 4 min 40% B, 4 -12 min, linear ramping up to 100% B, 12 – 18 min 100% B, 19 - 24 min 10% B, 24.1 min stop. The flow rate was 250 µL/min, and the column temperature was controlled at 40 ºC. |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Raptor Biphenyl (150 × 2.1 mm,2.7um) |
| Column Pressure: | 2800 psi - 4200 psi |
| Column Temperature: | 40 |
| Flow Gradient: | Constant |
| Flow Rate: | 250 µL/min |
| Injection Temperature: | 4 |
| Sample Injection: | 10 uL |
| Solvent A: | 90% water/10% methanol; 0.1% formic acid, pH 4.0 |
| Solvent B: | 50% methanol/50% isopropanol; 0.1% formic acid |
| Analytical Time: | 25 min |
| Oven Temperature: | 40 |
| Sample Loop Size: | 10 |
| Sample Syringe Size: | 100 |
| Chromatography Type: | Reversed phase |
