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MB Sample ID: SA167166
| Local Sample ID: | HFD_7 |
| Subject ID: | SU001872 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6/J |
| Age Or Age Range: | 6-7 weeks |
| Gender: | Male |
| Animal Housing: | temperature-controlled room under specific-pathogen free (SPF) or standard animal housing conditions with free access to food and water |
| Animal Feed: | semi-purified normal chow diet (control fat diet (CFD), 7% w/w fat and 16% total energy from fat; AIN93G, Specialty Feeds Pty Ltd, Australia) or high fat diet (HFD, 36% w/w fat and 59% total energy from fat; SF03-002, Specialty Feeds Pty Ltd, Australia) |
| Animal Water: | ad libitum |
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Combined analysis:
| Analysis ID | AN002915 | AN002916 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
| Column | Sigma Aldrich,Supleco,C8 (100 x 2.1mm,2.7um) | Sigma Aldrich,Supleco,C8 (100 x 2.1mm,2.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | height | height |
Chromatography:
| Chromatography ID: | CH002159 |
| Chromatography Summary: | Lipidomics analysis was performed using reversed phase liquid chromatography and high-resolution mass spectrometry. Samples (10 µl) were injected onto a Dionex Ultimate 3000 UHPLC system (Thermo Scientific, Australia) fitted with an analytical C8 column (100 x 2.1 mm; 2.7 µm, Sigma Aldrich, Australia). Chromatography was performed using solvent A (2 mM formic acid, 8 mM ammonium formate, 40% v/v isopropanol) and solvent B (2 mM formic acid, 8 mM ammonium formate, 98% v/v isopropanol) as mobile phases with a 30 min gradient starting at 0% B and increasing to 35% B from 0 to 8 min, then to 50% B from 8-16 min, then to 80% B from 16-19 min, then finally to 100% B by 23 min. 100% B was maintained for a further 3 min before equilibrating to 0% by 28 min and washing for a further 2 min. |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Sigma Aldrich,Supleco,C8 (100 x 2.1mm,2.7um) |
| Column Temperature: | 40C |
| Flow Gradient: | 0 min - 0%B, 8 min - 35%B, 16 min - 50%B, 19 min - 80%B, 23 min 100%B, 26 min - 100%B, 28 min - 0%B |
| Flow Rate: | 0.2 ml/min |
| Injection Temperature: | 4 |
| Solvent A: | 40% isopropanol/60% water; 2 mM formic acid; 8 mM ammonium formate, |
| Solvent B: | 98% isopropanol/2% water; 2 mM formic acid; 8 mM ammonium formate |
| Analytical Time: | 30 min |
| Oven Temperature: | 40C |
| Sample Loop Size: | 25 uL |
| Sample Syringe Size: | 25 uL |
| Chromatography Type: | Reversed phase |
