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MB Sample ID: SA169168
| Local Sample ID: | RBP42_WT_1 |
| Subject ID: | SU001899 |
| Subject Type: | Cultured cells |
| Subject Species: | Trypanosoma brucei brucei |
| Taxonomy ID: | 5702 |
| Genotype Strain: | 427 |
| Species Group: | Unicellular parasites |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002958 |
|---|---|
| Chromatography ID | CH002191 |
| MS ID | MS002748 |
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | NEGATIVE |
| Units | arbitrary |
Chromatography:
| Chromatography ID: | CH002191 |
| Chromatography Summary: | HILIC separation was performed on a Vanquish Horizon UHPLC system (Thermo Fisher Scientific, Waltham, MA) with an XBridge BEH Amide column (150 mm × 2.1 mm, 2.5 μm particle size, Waters, Milford, MA) using a gradient of solvent A (95%:5% H2O:acetonitrile with 20 mM acetic acid, 40 mM ammonium hydroxide, pH 9.4) and solvent B (20%:80% H2O:acetonitrile with 20 mM acetic acid, 40 mM ammonium hydroxide, pH 9.4). The gradient was 0 min, 100% B; 3 min, 100% B; 3.2 min, 90% B; 6.2 min, 90% B; 6.5 min, 80% B; 10.5 min, 80% B; 10.7 min, 70% B; 13.5 min, 70% B; 13.7 min, 45% B; 16 min, 45% B; 16.5 min, 100% B; and 22 min, 100% B. The flow rate was 300 μl/min. The injection volume was 5 μL, and the column temperature was set to 25°C. The autosampler temperature was set to 4°C, and the injection volume was 5 µL. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 25 |
| Flow Gradient: | 0 min, 100% B; 3 min, 100% B; 3.2 min, 90% B; 6.2 min, 90% B; 6.5 min, 80% B; 10.5 min, 80% B; 10.7 min, 70% B; 13.5 min, 70% B; 13.7 min, 45% B; 16 min, 45% B; 16.5 min, 100% B; and 22 min, 100% B. |
| Flow Rate: | 300 µl/min |
| Solvent A: | 95% water/5% acetonitrile; 20 mM acetic acid; 40 mM ammonium hydroxide, pH 9.4 |
| Solvent B: | 20% water/80% acetonitrile; 20 mM acetic acid, 40 mM ammonium hydroxide, pH 9.4 |
| Chromatography Type: | HILIC |
