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MB Sample ID: SA169210
| Local Sample ID: | 62 |
| Subject ID: | SU001900 |
| Subject Type: | Mammal |
| Subject Species: | Equus caballus |
| Taxonomy ID: | 9796 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002959 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Xcalibur |
| Column | Phenomenex Synergi Fusion (150mm x 2mm,4um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE |
| Units | peak intensity |
Chromatography:
| Chromatography ID: | CH002192 |
| Chromatography Summary: | Samples were maintained at 4 °C before injection. The injection volume was 10 µL. Chromatographic separation was achieved on a Synergi Fusion 4µm, 150 mm x 2 mm reverse phase column (Phenomenex, Torrance, CA) maintained at 30 °C using a solvent gradient method. Solvent A was water (0.1% formic acid). Solvent B was methanol (0.1% formic acid). The gradient method used was 0-5 min (10% B to 40% B), 5-7 min (40% B to 95% B), 7-9 min (95% B), 9-9.1 min (95% B to 10% B), 9.1-13 min (10% B). The flow rate was 0.4 mL min-1. |
| Instrument Name: | Thermo Xcalibur |
| Column Name: | Phenomenex Synergi Fusion (150mm x 2mm,4um) |
| Column Temperature: | 30 |
| Flow Gradient: | 0-5 min (10% B to 40% B), 5-7 min (40% B to 95% B), 7-9 min (95% B), 9-9.1 min (95% B to 10% B), 9.1-13 min (10% B). |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
