Return to study ST001866 main page
MB Sample ID: SA174497
| Local Sample ID: | PHI_6 |
| Subject ID: | SU001943 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003026 | AN003027 | AN003028 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | Unspecified | Unspecified |
| Chromatography system | Thermo-Finnigan Trace DSQ | Waters Acquity | Waters Acquity |
| Column | per Metabolon | per Metabolon | per Metabolon |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Single quadrupole | Single quadrupole | Single quadrupole |
| MS instrument name | Thermo LTQ-FT | Thermo LTQ-FT | Thermo LTQ-FT |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
| Units | Peak | Peak | Peak |
Chromatography:
| Chromatography ID: | CH002242 |
| Chromatography Summary: | The samples destined for GC/MS analysis were re-dried under vacuum desiccation for a minimum of 24 hours prior to being derivatized under dried nitrogen using bistrimethyl-silyl-triflouroacetamide (BSTFA). The GC column was 5% phenyl and the temperature ramp is from 40° to 300° C in a 16 minute period. Samples were analyzed on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer using electron impact ionization. The instrument was tuned and calibrated for mass resolution and mass accuracy on a daily basis. |
| Instrument Name: | Thermo-Finnigan Trace DSQ |
| Column Name: | per Metabolon |
| Chromatography Type: | GC |
| Chromatography ID: | CH002243 |
| Chromatography Summary: | The LC/MS portion of the platform was based on a Waters ACQUITY UPLC and a Thermo-Finnigan LTQ mass spectrometer, which consisted of an electrospray ionization (ESI) source and linear ion-trap (LIT) mass analyzer. The sample extract was split into two aliquots, dried, then reconstituted in acidic or basic LC-compatible solvents, each of which contained 11 or more injection standards at fixed concentrations. One aliquot was analyzed using acidic positive ion optimized conditions and the other using basic negative ion optimized conditions in two independent injections using separate dedicated columns. Extracts reconstituted in acidic conditions were gradient eluted using water and methanol both containing 0.1% Formic acid, while the basic extracts, which also used water/methanol, contained 6.5mM Ammonium Bicarbonate. |
| Instrument Name: | Waters Acquity |
| Column Name: | per Metabolon |
| Chromatography Type: | Unspecified |
