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MB Sample ID: SA177926
| Local Sample ID: | MAC3 |
| Subject ID: | SU002000 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003123 | AN003124 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
| Column | Phenomenex Luna NH2 (150 x 2.1mm,3um) | Waters Acquity BEH C18 (150 x 2mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED | POSITIVE |
| Units | counts | counts |
Chromatography:
| Chromatography ID: | CH002308 |
| Chromatography Summary: | For HILIC LC-MS/MS analysis, samples were analysed in positive and negative ion mode using a Phenomenex Luna NH2 analytical column (100 mm x 2 mm, 3 µm) held at room temperature with 10 minute linear gradient (A - 5% MeCN/20mM NH4CH2CO2/20mM NH4OH; B - MeCN) from 95 to 0 % MeCN followed by 5 minute hold at a flow rate of 0.400 mL/min. |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH002309 |
| Chromatography Summary: | For reverse phase LC-MS/MS analysis, samples were analysed in positive ion mode only using a Waters Acquity BEH C18 analytical column (100 mm x 2.1 mm, 1.7 µm) held at 40oC with 4 minute linear gradient (A - H2O + 0.1% formic acid; B - MeOH + 0.1% formic acid) from 0.5 to 70% MeOH followed by a 5 minute hold at a flow rate of 0.350 mL/min. |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Waters Acquity BEH C18 (150 x 2mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 4 minute linear gradient from 0.5 to 70% MeOH followed by a 5 minute hold |
| Flow Rate: | 0.350 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
