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MB Sample ID: SA181890
| Local Sample ID: | Normal-Liver-3 |
| Subject ID: | SU002013 |
| Subject Type: | Mammal |
| Subject Species: | Macaca fascicularis |
| Taxonomy ID: | 9541 |
| Age Or Age Range: | 16.3 - 18.8 yo |
| Weight Or Weight Range: | 5.73 - 10.56 kg |
| Gender: | Male |
| Animal Animal Supplier: | Guangzhou Huazhen Biosciences Co., Ltd, Guangzhou |
| Animal Housing: | Guangzhou Huazhen Biosciences Co., Ltd, Guangzhou |
| Animal Light Cycle: | 12-h/12-h light-dark cycle |
| Animal Feed: | normal food (19.26 % protein, 5% fat, no sugar, and no cholesterol), or high-fat and high-sugar (HFHS) food (≥ 30% sugar, ≥ 15% fat, ≥ 10.5% protein, and ≥ 0.5% cholesterol) |
| Animal Inclusion Criteria: | spontaneous diabetes mellitus and HFHS food-fed |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003145 | AN003146 | AN003147 | AN003148 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Shim-pack UFLC SHIMADZU CBM A system | Shim-pack UFLC SHIMADZU CBM A system | Shim-pack UFLC SHIMADZU CBM A system | Shim-pack UFLC SHIMADZU CBM A system |
| Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | EI | EI | EI | EI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH002327 |
| Chromatography Summary: | The analysis of hydrophilic metabolites used 0.1% formic acid (in water) and acetonitrile with 0.1% formic acid as mobile phases, with a gradient (V/V) as following: 95:5 at 0 min, 10:90 at 11.0 min, 10:90 at 12.0 min, 95:5 at 12.1 min, and 95:5 at 14.0 min. |
| Instrument Name: | Shim-pack UFLC SHIMADZU CBM A system |
| Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Flow Gradient: | 95:5 at 0 min, 10:90 at 11.0 min, 10:90 at 12.0 min, 95:5 at 12.1 min, and 95:5 at 14.0 min. |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002328 |
| Chromatography Summary: | The two mobile phases for hydrophobic compounds were 0.04% acetic acid and acetonitrile with 0.04% acetic acid, and the gradient (V/V) program was 95:5 at 0 min, 5:95 at 11.0 min, 5:95 at 12.0 min, 95:5 at 12.1 min and 95:5 at 14.0 min. |
| Instrument Name: | Shim-pack UFLC SHIMADZU CBM A system |
| Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Flow Gradient: | 95:5 at 0 min, 5:95 at 11.0 min, 5:95 at 12.0 min, 95:5 at 12.1 min and 95:5 at 14.0 min. |
| Solvent A: | 100% water; 0.04% acetic acid |
| Solvent B: | 100% acetonitrile; 0.04% acetic acid |
| Chromatography Type: | Reversed phase |
