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MB Sample ID: SA186791
| Local Sample ID: | NecJ 8h 3 |
| Subject ID: | SU002077 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003255 | AN003256 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) | Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH002399 |
| Chromatography Summary: | 10 uL of each sample was injected. The LC was operated under isocratic conditions using 95% phase A (0.1% formic acid in water) and 5% phase B (0.1% formic acid in acetonitrile) at 250 uL/min. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) |
| Column Temperature: | 25 |
| Flow Gradient: | isocratic |
| Flow Rate: | 250 ul/min |
| Solvent A: | 5% acetonitrile/95%water; 0.1% formic acid |
| Solvent B: | isocratic |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002400 |
| Chromatography Summary: | 10 uL of each sample was injected. The LC was operated under isocratic conditions using phase A (95% water, 5% acetonitrile, 10 mM ammonium acetate). |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) |
| Column Temperature: | 25 |
| Flow Gradient: | isocratic |
| Flow Rate: | 250 ul/min |
| Solvent A: | 5% acetonitrile/95%water; 0.1% formic acid |
| Solvent B: | isocratic |
| Chromatography Type: | Reversed phase |
