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MB Sample ID: SA188686
| Local Sample ID: | Pat_21_NEG |
| Subject ID: | SU002097 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003284 | AN003285 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Accucore C18 (100 x 2.1mm,2.6um) | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Orbitrap ID-X tribrid | Thermo Orbitrap ID-X tribrid |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | intensity | intensity |
Chromatography:
| Chromatography ID: | CH002425 |
| Chromatography Summary: | Samples were injected in randomized fashion via a Thermo Vanquish UHPLC and separation of the polar metabolites was achieved using Thermo Accucore C18 column (Thermo Scientific; 2.1 x 100 mm; 1.5 µm) maintained at 30 °C. The injection volume was 10 µL. For the 15-minute gradient, the standard mobile phase for RPLC was A = 0.1% formic acid in water and B = 0.1% formic acid in methanol. The linear elution gradient was as follows: 0-8.0 minutes at 50% B, 8.0 – 13.0 minutes held at 98% B, 13.1 to 15.0 minutes revert to 0% B to re-equilibration for next injection at a flow rate of 0.25 mL/min. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| Column Temperature: | 30C |
| Flow Gradient: | 0-8 minutes 50% B, 8 - 13 minutes held at 98% B, 13.1 to 15.0 minutes revert to 0% B to re-equilibration for next injection |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
