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MB Sample ID: SA193605

Local Sample ID:0424_PP_Tachy_Tubes_T1_3
Subject ID:SU002133
Subject Type:Other organism
Subject Species:Toxoplasma gondii
Taxonomy ID:5811

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Combined analysis:

Analysis ID AN003338
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Exactive Plus Orbitrap
Ion Mode UNSPECIFIED
Units counts

Chromatography:

Chromatography ID:CH002472
Chromatography Summary:Chromatographic separation was achieved on a Vanquish Flex fitted with an ACQUITY UPLC BEH Amide column (Waters). Running a 25 min linear gradient starting with 90 % eluent A (10 mM ammonium carbonate in acetonitrile) / 10 % eluent B (10 mM ammonium carbonate in water) and ending with 40 % eluent A / 60 % eluent B, followed by washing and equilibration steps.
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Flow Gradient:Running a 25 min linear gradient starting with 90% A / 10% B and ending with 40% eluent A / 60% eluent B, followed by washing and equilibration steps.
Solvent A:100% acetonitrile; 10 mM ammonium carbonate
Solvent B:100% water; 10 mM ammonium carbonate
Chromatography Type:HILIC
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