Return to study ST002179 main page
MB Sample ID: SA209269
| Local Sample ID: | Hgd80.3 - No treatment NEG |
| Subject ID: | SU002265 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003568 | AN003569 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent Infinity II | Agilent Infinity II |
| Column | Atlantis dC18 (3.0x100mm,3m,Waters,UK) | Atlantis dC18 (3.0x100mm,3m,Waters,UK) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | area | area |
Chromatography:
| Chromatography ID: | CH002639 |
| Chromatography Summary: | Liquid chromatography (LC) was performed on an Agilent 1290 Infinity II LC system. An Atlantis dC18 column (3.0x100mm, 3µm, Waters, UK) was maintained at 60°C with a flow rate of 0.4mL/min. Mobile phases were (A) water and (B) methanol both containing 5mmol/L ammonium formate and 0.1% formic acid. The elution gradient started at 5% mobile phase B at 0-1 min increasing linearly to 100% B by 12 min, held at 100% B until 14 min, returning to 95% A for 5 min to recondition the column. Injection volume was 1µL. |
| Instrument Name: | Agilent Infinity II |
| Column Name: | Atlantis dC18 (3.0x100mm,3m,Waters,UK) |
| Column Temperature: | 60 |
| Flow Gradient: | The elution gradient started at 5% mobile phase B at 0-1 min increasing linearly to 100% B by 12 min, held at 100% B until 14 min, returning to 95% A for 5 min to recondition the column |
| Flow Rate: | 0.4mL/min |
| Solvent A: | 100% water; 0.1% formic acid; 5 mM ammonium formate |
| Solvent B: | 100% acetonitrile; 0.1% formic acid;5 mM ammonium formate |
| Chromatography Type: | Reversed phase |
