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MB Sample ID: SA210336
| Local Sample ID: | CNMI_24 |
| Subject ID: | SU002280 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003591 | AN003592 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | GC |
| Chromatography system | Agilent 7100 CE | 8890 |
| Column | silica capillary (100 cm; inner diameter,50um,Agilent Technologies) | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | ESI | EI |
| MS instrument type | TOF | Single quadrupole |
| MS instrument name | Agilent CE-TOFMS | Agilent 5977B |
| Ion Mode | POSITIVE | UNSPECIFIED |
| Units | Peak Area | Corrected areas |
Chromatography:
| Chromatography ID: | CH002653 |
| Chromatography Summary: | Metabolite separation was performed in a fused silica capillary (100 cm; inner diameter, 50 µm, Agilent Technologies). Before each analysis, background electrolyte (BFE) (0.8 M formic acid solution in 10 % MeOH; v/v) was flushed for 5 min (950 mbar). Samples injections were performed over 50 s at 50 mbar and BGE was injected after each injection for 10 s at 100 mbar to improve reproducibility. The separation was carried out with an internal pressure of 25 mbar and 30 kV voltage with a total analytical run time of 35 min. |
| Methods Filename: | Sample_Analysis.pdf |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | silica capillary (100 cm; inner diameter,50um,Agilent Technologies) |
| Chromatography Type: | CE |
| Chromatography ID: | CH002654 |
| Chromatography Summary: | The flow rate of helium carrier gas was constant at 1.1359 mL/min and the injector temperature was set at 250 °C. The lock of the retention time (RTL) relative to the internal standard (methyl stearate) peak at 19.66 min was performed. The oven temperature gradient was initially set at 60 °C and was maintained for 1 min. Then it was raised by 10 °C/min until it reached 325 °C, and then was held at this temperature for 10 min before cooling down. The total analysis run time was 37.5 min. |
| Methods Filename: | Sample_Analysis.pdf |
| Instrument Name: | 8890 |
| Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| Chromatography Type: | GC |
