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MB Sample ID: SA211367
| Local Sample ID: | S4 |
| Subject ID: | SU002290 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | wild type and endothelium-specific Sirt1 knockout mice |
| Age Or Age Range: | 12 weeks old |
| Weight Or Weight Range: | ~30 g |
| Height Or Height Range: | n/a |
| Gender: | Male |
| Animal Animal Supplier: | Jax |
| Animal Housing: | University of Iowa animal house |
| Animal Light Cycle: | 12 h light/12 h night |
| Animal Feed: | Standard Chow |
| Animal Water: | Tap water |
| Animal Inclusion Criteria: | n/a |
| Species Group: | n/a |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003607 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Thermo Trace 1310 |
| Column | Thermo TraceGold TG-5SilMS |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Thermo ISQ |
| Ion Mode | POSITIVE |
| Units | AU |
Chromatography:
| Chromatography ID: | CH002666 |
| Chromatography Summary: | 1 μl of derivatized sample was injected into a Trace 1300 GC (Thermo) fitted with a TraceGold TG-5SilMS column (Thermo) operating under the following conditions: split ratio = 20-1, split flow = 24 μl/minute, purge flow = 5 ml/minute, carrier mode = Constant Flow, and carrier flow rate = 1.2 ml/minute. The GC oven temperature gradient was as follows: 80°C for 3 minutes, increasing at a rate of 20°C/minute to 280°C, and holding at a temperature at 280°C for 8 minutes. |
| Methods Filename: | GC_Method_Tissue.pdf |
| Instrument Name: | Thermo Trace 1310 |
| Column Name: | Thermo TraceGold TG-5SilMS |
| Chromatography Type: | GC |
