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MB Sample ID: SA211368
Local Sample ID: | S2 |
Subject ID: | SU002290 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | wild type and endothelium-specific Sirt1 knockout mice |
Age Or Age Range: | 12 weeks old |
Weight Or Weight Range: | ~30 g |
Height Or Height Range: | n/a |
Gender: | Male |
Animal Animal Supplier: | Jax |
Animal Housing: | University of Iowa animal house |
Animal Light Cycle: | 12 h light/12 h night |
Animal Feed: | Standard Chow |
Animal Water: | Tap water |
Animal Inclusion Criteria: | n/a |
Species Group: | n/a |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003607 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Thermo Trace 1310 |
Column | Thermo TraceGold TG-5SilMS |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Thermo ISQ |
Ion Mode | POSITIVE |
Units | AU |
Chromatography:
Chromatography ID: | CH002666 |
Chromatography Summary: | 1 μl of derivatized sample was injected into a Trace 1300 GC (Thermo) fitted with a TraceGold TG-5SilMS column (Thermo) operating under the following conditions: split ratio = 20-1, split flow = 24 μl/minute, purge flow = 5 ml/minute, carrier mode = Constant Flow, and carrier flow rate = 1.2 ml/minute. The GC oven temperature gradient was as follows: 80°C for 3 minutes, increasing at a rate of 20°C/minute to 280°C, and holding at a temperature at 280°C for 8 minutes. |
Methods Filename: | GC_Method_Tissue.pdf |
Instrument Name: | Thermo Trace 1310 |
Column Name: | Thermo TraceGold TG-5SilMS |
Chromatography Type: | GC |