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MB Sample ID: SA229453
| Local Sample ID: | Blank 7 |
| Subject ID: | SU002414 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003796 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Dionex Ultimate 3000TM |
| Column | Millipore(TM) ZIC-pHILIC |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | intensity |
Chromatography:
| Chromatography ID: | CH002809 |
| Chromatography Summary: | The LC column was a Millipore TMZIC-pHILIC (2.1x150 mm, 5μm) coupled to a Dionex Ultimate 3000TM system and the column oven temperature was set to 25oC for the gradient elution. A flow rate of 100μL/min was used with the 10 mM ammonium carbonate in water (A), pH 9.0, and acetonitrile (B). The gradient profile was 80-20% B (0-30min), 20-80% B (30-31min), 80-80% B (31-42min). Injection volume was set to 2 μL for all analyses (42min total run time per injection). |
| Methods Filename: | metabolomics_methods.pdf |
| Instrument Name: | Dionex Ultimate 3000TM |
| Column Name: | Millipore(TM) ZIC-pHILIC |
| Column Temperature: | 25 |
| Flow Gradient: | 80-20% B (0-30min), 20-80% B (30-31min), 80-80% B (31-42min). |
| Flow Rate: | 100µL/min |
| Solvent A: | 100% water; 10 mM ammonium carbonate, pH 9.0 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
