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MB Sample ID: SA229467
Local Sample ID: | Blank 6 |
Subject ID: | SU002414 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003796 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Dionex Ultimate 3000TM |
Column | Millipore(TM) ZIC-pHILIC |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE |
Units | intensity |
Chromatography:
Chromatography ID: | CH002809 |
Chromatography Summary: | The LC column was a Millipore TMZIC-pHILIC (2.1x150 mm, 5μm) coupled to a Dionex Ultimate 3000TM system and the column oven temperature was set to 25oC for the gradient elution. A flow rate of 100μL/min was used with the 10 mM ammonium carbonate in water (A), pH 9.0, and acetonitrile (B). The gradient profile was 80-20% B (0-30min), 20-80% B (30-31min), 80-80% B (31-42min). Injection volume was set to 2 μL for all analyses (42min total run time per injection). |
Methods Filename: | metabolomics_methods.pdf |
Instrument Name: | Dionex Ultimate 3000TM |
Column Name: | Millipore(TM) ZIC-pHILIC |
Column Temperature: | 25 |
Flow Gradient: | 80-20% B (0-30min), 20-80% B (30-31min), 80-80% B (31-42min). |
Flow Rate: | 100µL/min |
Solvent A: | 100% water; 10 mM ammonium carbonate, pH 9.0 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |