Return to study ST002458 main page
MB Sample ID: SA246074
| Local Sample ID: | 09_KO_Rapha3 |
| Subject ID: | SU002548 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004010 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Merck SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | concentration |
Chromatography:
| Chromatography ID: | CH002961 |
| Chromatography Summary: | UPLC setup consisted a ZIC-pHILIC column (SeQuant; 150 mm × 2.1 mm, 5 μm; Merck). 5 µl of cells or kidney extracts were injected using an autosampler. Compounds were separated using a 15 minutes gradient, starting at 20% aqueous (20 mM ammonium carbonate adjusted to pH 9.2 with 0.1% of 25% ammonium hydroxide) and 80% organic (acetonitrile), terminated with 20% acetonitrile. Flow rate and column temperature were kept at 0.2 ml/min and 45°C, respectively for a total run time of 27 min |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Merck SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
| Column Temperature: | 45 |
| Flow Gradient: | 15 min terminated with 20% acetonitrile |
| Flow Rate: | 0.2 ml/min |
| Solvent A: | 20% water/80% acetonitrile; 20 mM ammonium carbonate; 0.1% of 25% ammonium hydroxide (adjusted to pH 9.2) |
| Solvent B: | 80%water/20% acetonitrile |
| Chromatography Type: | HILIC |
