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MB Sample ID: SA247107
| Local Sample ID: | uri_sdm_8_M_3 |
| Subject ID: | SU002555 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 3-12 weeks |
| Animal Animal Supplier: | In house breeding |
| Animal Housing: | Germ-free and gnotobiotic isolators for germ and gnotobiotic mice. Individually ventilated cages for specific pathogen free mice |
| Animal Light Cycle: | 12 hours |
| Animal Feed: | LabDiet (Cat# 5010; Laboratory Autoclavable Rodent Diet) |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004021 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Thermo Syncronis HILIC (100 x 2.1mm, 1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | NEGATIVE |
| Units | AUC |
Chromatography:
| Chromatography ID: | CH002971 |
| Chromatography Summary: | All metabolomics data were collected at the Calgary Metabolomics Research Facility (CMRF). Briefly, samples were centrifuged (20,817 xg, 15 minutes, 4°C), then 200µL was transferred to a deep-well 96-well plate (Thermo FisherF) for LC-MS analysis. Data were collected on a Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ Mass Spectrometer (Thermo-Fisher) coupled to a Vanquish™ UHPLC System (Thermo-Fisher). Metabolites were chromatographically separated on Syncronis HILIC UHPLC column (2.1mm x 100mm x 1.7um, Thermo-Fisher) at the flow rate of 600uL/min using a binary solvent system (solvent A, 20mM ammonium formate pH 3.0 in MS grade H20 and solvent B, MS grade acetonitrile with 0.1% formic acid (%v/v)) and the following gradient: 0-2 mins, 100 %B; 2-7 mins, 100-80 %B; 7-10 mins, 80-5 %B; 10-12 mins, 5% B; 12-13 mins, 5-100 %B; 13-15 mins, 100 %B. The samples injection volume was 2uL. MassM data were acquired in MS1, negative full scan mode at a resolution of 240,000 scanning from 50-750m/z. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Syncronis HILIC (100 x 2.1mm, 1.7um) |
| Column Temperature: | 30C |
| Flow Gradient: | 2 stage linear gradient |
| Flow Rate: | 600uL/min |
| Sample Injection: | 2uL |
| Solvent A: | 100% water; 20mM ammonium formate (pH3) |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 15 minutes |
| Washing Buffer: | 10% MeOH in H2O + 0.1% formic acid |
| Target Sample Temperature: | 4 |
| Chromatography Type: | HILIC |
