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MB Sample ID: SA247997
| Local Sample ID: | PMN_28 |
| Subject ID: | SU002567 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004046 |
|---|---|
| Chromatography ID | CH002993 |
| MS ID | MS003793 |
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Scientific QExactive HF-X hybrid quadrupole orbitrap high-resolution mass spectrometer (HRMS) |
| Column | Millipore ZIC-pHILIC column (5 mm, 2.1×150 mm) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | MS reading |
Chromatography:
| Chromatography ID: | CH002993 |
| Chromatography Summary: | Liquid chromatography was performed using a Millipore ZIC-pHILIC column (5 mm, 2.1×150 mm) with a binary solvent system of 10 mM ammonium acetate in water, pH 9.8 (solvent A) and acetonitrile (solvent B) with a constant flow rate of 0.25 mL/min. The column was equilibrated with 90% solvent B. The liquid chromatography gradient was: 0–15 minutes linear ramp from 90% B to 30% B; 15–18 minutes isocratic flow of 30% B; 18–19 minutes linear ramp from 30% B to 90% B; 19–27 column regeneration with isocratic flow of 90% B. |
| Instrument Name: | Thermo Scientific QExactive HF-X hybrid quadrupole orbitrap high-resolution mass spectrometer (HRMS) |
| Column Name: | Millipore ZIC-pHILIC column (5 mm, 2.1×150 mm) |
| Column Temperature: | 40 |
| Flow Gradient: | 0–15 minutes linear ramp from 90% B to 30% B; 15–18 minutes isocratic flow of 30% B; 18–19 minutes linear ramp from 30% B to 90% B; 19–27 column regeneration with isocratic flow of 90% B. |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 100% water; 10 mM ammonium acetate, pH 9.8 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
