Return to study ST002352 main page

MB Sample ID: SA249746

Local Sample ID:MED1 Aqueous Sample 8
Subject ID:SU002592
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN004097 AN004098
Analysis type MS MS
Chromatography type Normal phase Normal phase
Chromatography system Agilent Model 1290 Infinity II liquid chromatography system Agilent Model 1290 Infinity II liquid chromatography system
Column Cogent Diamond Hydride (150 × 2.1 mm, 4um) Cogent Diamond Hydride (150 × 2.1 mm, 4um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6550 QTOF Agilent 6550 QTOF
Ion Mode POSITIVE NEGATIVE
Units Ion counts Ion counts

Chromatography:

Chromatography ID:CH003034
Chromatography Summary:Tissue extracts were analyzed by LC/MS as described previously, using a platform comprised of an Agilent Model 1290 Infinity II liquid chromatography system coupled to an Agilent 6550 iFunnel time-of-flight MS analyzer. Chromatography of metabolites utilized aqueous normal phase (ANP) chromatography on a Diamond Hydride column (Microsolv). Mobile phases consisted of: (A) 50% isopropanol, containing 0.025% acetic acid, and (B) 90% acetonitrile containing 5 mM ammonium acetate. To eliminate the interference of metal ions on chromatographic peak integrity and electrospray ionization, EDTA was added to the mobile phase at a final concentration of 5 µM. The following gradient was applied: 0-1.0 min, 99% B; 1.0-15.0 min, to 20% B; 15.0 to 29.0, 0% B; 29.1 to 37min, 99% B.
Instrument Name:Agilent Model 1290 Infinity II liquid chromatography system
Column Name:Cogent Diamond Hydride (150 × 2.1 mm, 4um)
Solvent A:50% isopropanol, containing 0.025% acetic acid
Solvent B:90% acetonitrile containing 5 mM ammonium acetate
Chromatography Type:Normal phase
  logo