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MB Sample ID: SA249756
Local Sample ID: | MED1 Input Sample 4 |
Subject ID: | SU002592 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Combined analysis:
Analysis ID | AN004097 | AN004098 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Normal phase | Normal phase |
Chromatography system | Agilent Model 1290 Infinity II liquid chromatography system | Agilent Model 1290 Infinity II liquid chromatography system |
Column | Cogent Diamond Hydride (150 × 2.1 mm, 4um) | Cogent Diamond Hydride (150 × 2.1 mm, 4um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Ion counts | Ion counts |
Chromatography:
Chromatography ID: | CH003034 |
Chromatography Summary: | Tissue extracts were analyzed by LC/MS as described previously, using a platform comprised of an Agilent Model 1290 Infinity II liquid chromatography system coupled to an Agilent 6550 iFunnel time-of-flight MS analyzer. Chromatography of metabolites utilized aqueous normal phase (ANP) chromatography on a Diamond Hydride column (Microsolv). Mobile phases consisted of: (A) 50% isopropanol, containing 0.025% acetic acid, and (B) 90% acetonitrile containing 5 mM ammonium acetate. To eliminate the interference of metal ions on chromatographic peak integrity and electrospray ionization, EDTA was added to the mobile phase at a final concentration of 5 µM. The following gradient was applied: 0-1.0 min, 99% B; 1.0-15.0 min, to 20% B; 15.0 to 29.0, 0% B; 29.1 to 37min, 99% B. |
Instrument Name: | Agilent Model 1290 Infinity II liquid chromatography system |
Column Name: | Cogent Diamond Hydride (150 × 2.1 mm, 4um) |
Solvent A: | 50% isopropanol, containing 0.025% acetic acid |
Solvent B: | 90% acetonitrile containing 5 mM ammonium acetate |
Chromatography Type: | Normal phase |