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MB Sample ID: SA250389

Local Sample ID:1-3
Subject ID:SU002596
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

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Combined analysis:

Analysis ID AN004106
Chromatography ID CH003041
MS ID MS003853
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000
Column Waters XBridge C18 (50 x 2.1mm,3um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Exactive Plus Orbitrap
Ion Mode NEGATIVE
Units peak area normalized to IS and cell number

Chromatography:

Chromatography ID:CH003041
Chromatography Summary:The sample run order was randomized to reduce bias from instrument drift. 10 µl sample was subjected to LC-MS analysis on a Exactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific) coupled to an Ultimate 3000 UHPLC system (Thermo Fisher Scientific). Reversed-phase chromatography mode was used to separate compounds on a Xselect C18 HSS column (Waters) with solvent A (97:3 water:methanol (v/v), 10 mM tributylamine, and 15 mM acetic acid ) and solvent B (methanol). The flow rate was 200 µl/min, and the total run time was 25 min. The gradient was 0 min, 0% B; 5 min, 20% B; 7.5 min, 55% B; 15 min, 65% B; 17.5 min, 95% B; and 21 min, 0% B. The mass spectrometer was operated in a negative-ion mode at a resolution of 140,000 at m/z 200 and with a scan range of 85 to 1000 m/z.
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters XBridge C18 (50 x 2.1mm,3um)
Column Temperature:standard
Flow Gradient:0 min, 0% B; 5 min, 20% B; 7.5 min, 55% B; 15 min, 65% B; 17.5 min, 95% B; and 21 min, 0% B.
Flow Rate:200 µl/min
Solvent A:97:3 water:methanol (v/v), 10 mM tributylamine, and 15 mM acetic acid
Solvent B:methanol
Chromatography Type:Reversed phase
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