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MB Sample ID: SA002844

Local Sample ID:MR2_CTL_271
Subject ID:SU000074
Subject Type:Animal cells
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:FLT3-ITD AML (MR2)
Cell Biosource Or Supplier:Dr. Tim Pardee (Wake Forest Univ.)
Cell Primary Immortalized:Primary
Species Group:Mammal

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Collection:

Collection ID:CO000057
Collection Summary:Conditioned Medias:Cellular Fraction_LC-MS:Cellular Fraction_WB
Collection Protocol Comments:Processed simultaneously/time point:Processed simultaneously/time point:Processed simultaneously/time point
Sample Type:conditioned treatment media
Collection Method:Total contents pipette-transferred to tubes & centrifuged at 1800 rpm for 5 minutes. Medias transferred into aliquot tubes.:Cellular fractions pelleted & resuspended in 1X PBS. Aliqotted into collection vials based on cell counts. Re-centrifuged at 8,000 rpm for 3 min at 4 °C. PBS was aspirated off pellets for storage.:Cellular fractions pelleted & resuspended in 1X PBS. Aliqotted into collection vial based on cell counts. Re-centrifuged at 8,000 rpm for 3 min at 4 °C. PBS was aspirated off pellets for storage.
Collection Location:RTI RCMRC Tissue culture suite:RTI RCMRC Wet lab:RTI RCMRC Wet lab
Collection Frequency:0h, 4h, 24h and 48h:0h, 4h, 24h and 48h:0h, 4h, 24h and 48h
Collection Duration:10 minutes:10 minutes:5 minutes
Collection Time:0h, 4h, 24h and 48h:0h, 4h, 24h and 48h:0h, 4h, 24h and 48h
Volumeoramount Collected:1 mL x 5 aliquots:2x10^7 cells:2x10^6 cells
Storage Conditions:-80C:-80C:-80C
Collection Vials:15 mL conical tubes:15 mL conical tubes:15 mL conical tubes
Storage Vials:1.5 mL Eppendorf Protein Lo-Bind tubes:1.5 mL Eppendorf Protein Lo-Bind tubes:1.5 mL Eppendorf Protein Lo-Bind tubes
Collection Tube Temp:chilled in ice:chilled in ice:chilled in ice
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