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MB Sample ID: SA008125
Local Sample ID: | 25D |
Subject ID: | SU000168 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
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Collection:
Collection ID: | CO000154 |
Collection Summary: | Percutaneous muscle biopsy specimens of the vastus lateralis (?300 mg) were performed at 180 and 480 minutes under local anesthesia (2% lidocaine). Muscle samples were blotted, were dissected free of fat and connective tissue, and then were frozen. Biopsy specimens were collected from the opposite legs on a single visit. Indirect calorimetry was performed at 11:00 am for 20 minutes using a ventilated hood (Parvo Medics). The final 10 minutes of steady-state oxygen consumption (Vo2) and carbon dioxide production (Vco2) determined the respiratory exchange ratio (RER = Vco2/Vo2). Urine was collected throughout the clamp and analyzed for urea content at a core laboratory. |
Sample Type: | Muscle |