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MB Sample ID: SA008199

Local Sample ID:5A
Subject ID:SU000168
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

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Collection:

Collection ID:CO000154
Collection Summary:Percutaneous muscle biopsy specimens of the vastus lateralis (?300 mg) were performed at 180 and 480 minutes under local anesthesia (2% lidocaine). Muscle samples were blotted, were dissected free of fat and connective tissue, and then were frozen. Biopsy specimens were collected from the opposite legs on a single visit. Indirect calorimetry was performed at 11:00 am for 20 minutes using a ventilated hood (Parvo Medics). The final 10 minutes of steady-state oxygen consumption (Vo2) and carbon dioxide production (Vco2) determined the respiratory exchange ratio (RER = Vco2/Vo2). Urine was collected throughout the clamp and analyzed for urea content at a core laboratory.
Sample Type:Muscle
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