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MB Sample ID: SA011313
| Local Sample ID: | 2219 |
| Subject ID: | SU000264 |
| Subject Type: | Animal |
| Subject Species: | Equus caballus |
| Taxonomy ID: | 9796 |
| Genotype Strain: | Arabian |
| Species Group: | Mammal |
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Collection:
| Collection ID: | CO000255 |
| Collection Summary: | A mobile set-up allowed for immediate processing of samples from study horses on their home farm. 50mL of blood was collected into two EDTA tubes, two no-additive tubes, and one sodium oxalate tube. EDTA and sodium oxalate treated samples were thoroughly mixed by inversion and immediately centrifuged to pellet the cells and allow for removal of plasma by pipette. Blood drawn in a no-additive tube was allowed to clot, centrifuged, and had the serum removed by pipette. The draw yielded 6-8 mL of plasma and 3-4mL of serum, which was then divided in to 1mL aliquots and immediately snap frozen by immersion in liquid nitrogen. Upon returning to the lab, the samples were then transfered to -80C for storage. Currently, only plasma is being submitted for metabolomic analysis. |
| Collection Protocol Filename: | collection.txt |
| Sample Type: | Blood |
| Collection Method: | jugular venipuncture |
| Collection Location: | On farm |
| Collection Duration: | 30 minutes per horse total |
| Collection Time: | na |
| Volumeoramount Collected: | 50ml whole blood |
| Storage Conditions: | snap frozen in LN2 on farm, -80 in lab |
| Collection Vials: | glass vacutainer |
| Storage Vials: | plastic cryovials |
| Collection Tube Temp: | ambient during draw and centrifugation, then LN2 after plasma removal |
| Additives: | EDTA |
| Blood Serum Or Plasma: | PLASMA |
