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MB Sample ID: SA013410

Local Sample ID:B1P505
Subject ID:SU000314
Subject Type:Insect
Subject Species:Sarcophaga crassipalpis
Taxonomy ID:59312
Genotype Strain:Lab colony
Age Or Age Range:15-25 day pupae
Weight Or Weight Range:110-130 mg
Animal Animal Supplier:lab colony
Animal Housing:sterilite containers
Animal Light Cycle:10:14 L:D
Animal Feed:beef liver
Animal Inclusion Criteria:must be in diapause
Species Group:Insect

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Collection:

Collection ID:CO000308
Collection Summary:Pupae were sampled at the appropriate time point and flash frozen in liquid nitrogen. Pupae were stored at -80C until extraction.
Collection Protocol Comments:Samples were homogenized in ice cold 80% methanol and centrifuged. Supernatant was retained and stored at -80C until analysis.
Sample Type:whole fly
Collection Method:flash freezing in liquid nitrogen
Collection Location:Hahn Lab, 3137 Steinmetz
Collection Frequency:Daily
Collection Time:see treatments
Volumeoramount Collected:one pupa per sample
Storage Conditions:-80C
Collection Vials:1.5 ml microcentrifuge tubes
Storage Vials:1.5 ml microcentrifuge tubes
Collection Tube Temp:room temperature
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