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MB Sample ID: SA018720
| Local Sample ID: | 061215byusa1115_1 |
| Subject ID: | SU000414 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl/6J |
| Age Or Age Range: | 4 months |
| Gender: | Female |
| Human Smoking Status: | Former/Current |
| Animal Animal Supplier: | Jackson Laboratories |
| Animal Housing: | housed in a facility at 23°C |
| Animal Light Cycle: | 12∶12 hr light cycle, lights on 0700 |
| Animal Feed: | with free access to chow (4.5% fat/weight), for at least 1 week before being studied |
| Species Group: | Mammal |
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Collection:
| Collection ID: | CO000408 |
| Collection Summary: | Skeletal muscle mitochondria were isolated essentially according to Chappell and Perry [Chappell JB, Perry SV (1954) Biochemical and osmotic properties of skeletal muscle mitochondria. Nature 173: 1094–1095.]. All media were ice-cold, and procedures done on ice or at 4°C. Briefly, pectoral, forelimb and hindlimb muscles were rapidly dissected and placed in basic medium [BM (mM): KCl (140), HEPES (20), MgCl2 (5), EGTA (2); pH 7.0]. Together, these muscle groups comprise a mixed population of mainly type II oxidative and glycolytic fibers. Muscle was cleaned of connective tissue and fat, minced and placed in 15 vol of homogenizing medium (HM: BM with 1 mM ATP and 1% BSA (w/v)) containing one unit of protease (Subtilisin A) per g muscle wet weight. |
| Collection Protocol Filename: | Long-Chain_Fatty_Acid_Metabolite_Profiles_in_Skeletal_Muscle_Mitochondria.PDF |
| Sample Type: | Mitochondria |
