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MB Sample ID: SA020438
| Local Sample ID: | ciMB1_B04a |
| Subject ID: | SU000435 |
| Subject Type: | Cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
| Genotype Strain: | 3D7 |
| Species Group: | Microorganism |
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Collection:
| Collection ID: | CO000429 |
| Collection Summary: | Asexual P. falciparum (3D7) were cultured with minor modifications to the method of Trager and Jensen using human RBC (Australian Red Cross Blood Service) at 3% hematocrit in modified RPMI medium containing hypoxanthine and 0.5% (w/v) albumax at 37 °C under defined atmosphere (95% N2, 4% CO2, 1% O2). Parasites were synchronized with 5% (w/v) sorbitol twice at an interval of 14 hours and cultured for a further 58 hours to ensure all experiments were performed on mid-trophozoite stage cultures (~30 h post infection) at 7-8% parasitaemia. |
| Sample Type: | Cell |
