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MB Sample ID: SA072842
| Local Sample ID: | PCC 7002 0 SEC REP 2 |
| Subject ID: | SU001124 |
| Subject Type: | Bacteria |
| Subject Species: | Synechococcus sp. PCC 7002 |
| Taxonomy ID: | 32049 |
| Genotype Strain: | Synechococcus |
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Collection:
| Collection ID: | CO001118 |
| Collection Summary: | The experiment was conducted in a reactor at a metabolic steady state (OD 730 nm = 0.8). 13C labeled sodium bicarbonate was used as a tracer. Tracer concentration was 1 g/L. After the introduction of the tracer, 15 mL of the culture were collected at time point : 0, 60, 120, 180 and 240 secs. Samples were quenched with methanol and extracted using methanol-chloroform-water method. Extracts were stored at -80 degree C till LCMS analysis. LCMS analysis was done in negative ion mode using information dependent aquisition (IDA) method. |
| Sample Type: | Bacterial cells |
