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MB Sample ID: SA077712
| Local Sample ID: | TBt0 plasma 711 |
| Subject ID: | SU001176 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO001170 |
| Collection Summary: | Isolation of tumor interstitial fluid (TIF) TIF was isolated from tumors using a previously described centrifugal method (Eil et al., 2016; Haslene-Hox et al., 2011; Ho et al., 2015; Wiig et al., 2003). Briefly, tumor bearing animals were euthanized by cervical dislocation and tumors were rapidly dissected from the animals. Dissections took <1 min. to complete. Blood was collected from the same animal via cardiac puncture, and was immediately placed in EDTA-tubes (Sarstedt, North Rhine-Westphalia, Germany) and centrifuged at 845 x g for 10 minutes at 4°C to separate plasma. Plasma was frozen in liquid nitrogen and stored at -80°C until further analysis. Tumors were then weighed and briefly rinsed in room temperature saline (150mM NaCl) and blotted on filter paper (VWR, Radnor, PA, 28298-020). The entire process of preparing the tumor prior to isolation of TIF took ~2 min. The tumors were then put onto 20µm nylon filters (Spectrum Labs, Waltham, MA, 148134) affixed atop 50mL conical tubes, and centrifuged for 10 min. at 4°C at 106 x g. TIF was then collected from the conical tube, frozen in liquid nitrogen and stored at -80°C until further analysis. |
| Sample Type: | Interstitial Fluid |
