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MB Sample ID: SA078045
| Local Sample ID: | BTSPT_2 |
| Subject ID: | SU001186 |
| Subject Type: | Bacteria |
| Subject Species: | Bacteroides thetaiotaomicron VPI-5482;Bacteroides ovatus ATCC 8483 |
| Taxonomy ID: | 226186;411476 |
| Genotype Strain: | WT (VPI-5482), SPT K.O. (BT_0870), (ATCC_8483), SPT K.O. (BACOVA_02588) |
| Age Or Age Range: | 411476 |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | Wild type Bacteroides strains and serine palmitoyltransferase (SPT) knockouts |
| Subject Comments: | Grown in BHI Media (Bacteroides tethaiotamicron, Bateroides ovatus : VPI-5482/ATCC_8483) |
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Collection:
| Collection ID: | CO001180 |
| Collection Summary: | WT and ΔSPT cultures of B. thetaiotaomicron and B. ovatus were grown in 5 mL of BHI liquid media supplemented with vitamin K and hemin. After 24 hrs, cultures were normalized by OD600 and centrifuged at 8,000 rpm for 10 min to pellet cells. Resulting pellets were washed and further centrifuged twice to remove residual media. Resulting pellets were resuspended in 5 mL isopropanol + 0.1 ng/μL C24:0 PC, incubated at RT for 1 hr, and centrifuged for 10 min at 10,000g. From each sample, 2 μL was injected. |
| Sample Type: | Bacterial cells |
| Storage Conditions: | -80℃ |
