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MB Sample ID: SA092004
| Local Sample ID: | FA |
| Subject ID: | SU001333 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | MCF-7 |
| Cell Passage Number: | 30 |
| Species Group: | Homo Sapien |
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Collection:
| Collection ID: | CO001327 |
| Collection Summary: | The breast adenocarcinoma cells (MCF-7) were cultured in DMEM medium supplemented with 10% fetal calf serum and 1% penicillin/streptomycin solution and incubated at 37oC in an atmosphere of 5% CO2. In preparation for an experiment, 3 × 106 cells were cultured in each of 15 tissue culture flasks (175 cm2) and cells were incubated for three days. When the cells reach 80% confluency, they were collected by trypsinization, counted in a cell counter and each 3 ×106 cells were suspended in 1 ml phosphate-buffered saline in an Eppendorf tube. |
| Sample Type: | Breast cancer cells |
| Collection Method: | trypsinization protocol |
| Storage Conditions: | -80℃ |
