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MB Sample ID: SA093453
Local Sample ID: | Cage7_Wk2 |
Subject ID: | SU001352 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl6 |
Age Or Age Range: | 5wks-13wks |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory (Bar Harbor, ME) |
Animal Light Cycle: | 12hr light 12hr dark |
Animal Feed: | LabDiet 5001 (PMI International, Inc, St. Louis, MO) ad libitum |
Animal Water: | ad libitum |
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Collection:
Collection ID: | CO001346 |
Collection Summary: | Urine: 18 hour collection from metabolic cages. At the end of each collection urine volume was measured and centrifuged (2000xg, 10 mins, °C). Internal standard was added equivalent to 10% of sample volume, aliquots were transferred into screw-top cryotubes and stored at -80°C. WB: cardiac puncture after anesthesia. Collected by cardiac puncture and transferred to a microcentrifuge tube containing sodium heparin in an ice water bath. Stored @ -80°C within 10 minutes of collection |
Collection Protocol Filename: | MouseUrineCollection,Processing_MetabolicCages.V1.5.pdf |
Collection Protocol Comments: | Urine Collected 4 times over 8 weeks, each collection over 18 hours |
Sample Type: | Urine |
Collection Method: | From Metabolic cages |
Collection Frequency: | 2 weeks |
Collection Duration: | 18hr |
Storage Conditions: | -80℃ |
Collection Vials: | Sterile 2mL microcentrifuge tubes |
Storage Vials: | Sterile 2mL microcentrifuge tubes |
Collection Tube Temp: | Room temp |