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MB Sample ID: SA093471

Local Sample ID:Cage3_Wk0
Subject ID:SU001352
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57Bl6
Age Or Age Range:5wks-13wks
Gender:Male
Animal Animal Supplier:Jackson Laboratory (Bar Harbor, ME)
Animal Light Cycle:12hr light 12hr dark
Animal Feed:LabDiet 5001 (PMI International, Inc, St. Louis, MO) ad libitum
Animal Water:ad libitum

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Collection:

Collection ID:CO001346
Collection Summary:Urine: 18 hour collection from metabolic cages. At the end of each collection urine volume was measured and centrifuged (2000xg, 10 mins, °C). Internal standard was added equivalent to 10% of sample volume, aliquots were transferred into screw-top cryotubes and stored at -80°C. WB: cardiac puncture after anesthesia. Collected by cardiac puncture and transferred to a microcentrifuge tube containing sodium heparin in an ice water bath. Stored @ -80°C within 10 minutes of collection
Collection Protocol Filename:MouseUrineCollection,Processing_MetabolicCages.V1.5.pdf
Collection Protocol Comments:Urine Collected 4 times over 8 weeks, each collection over 18 hours
Sample Type:Urine
Collection Method:From Metabolic cages
Collection Frequency:2 weeks
Collection Duration:18hr
Storage Conditions:-80℃
Collection Vials:Sterile 2mL microcentrifuge tubes
Storage Vials:Sterile 2mL microcentrifuge tubes
Collection Tube Temp:Room temp
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