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MB Sample ID: SA094327
| Local Sample ID: | 11801 LDC 43-HR BR1 IS IDA-2 |
| Subject ID: | SU001376 |
| Subject Type: | Bacteria |
| Subject Species: | Synechococcus elongatus PCC 11801 |
| Taxonomy ID: | 2219813 |
| Genotype Strain: | Synechococcus elongatus PCC 11801 |
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Collection:
| Collection ID: | CO001371 |
| Collection Summary: | Experiments were carried out by growing Synechococcus elongatus PCC 11801 cells in multicultivators designed to provide sinusoidal light. The period was 14:10 (light-dark). The samples for metabolomics analysis were collected in the second diurnal cycle at an interval of 6 hours starting from 25th hour (25, 31, 37 and 43 hours). The light intensity amplitude was 600 µmole photons m-2 s-1. Additionally samples were also collected using cells grown under continuous light illumination of 600 µmole photons m-2 s-1 to compare the difference in metabolite levels compared to that in the diurnal cycle. Samples were quenched with methanol and extracted using the methanol-chloroform-water method. Extracts were stored at -80°C till LCMS analysis. LCMS analysis was done in the negative ion mode using information-dependent acquisition (IDA) method. |
| Sample Type: | Bacterial cells |
