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MB Sample ID: SA094352
| Local Sample ID: | Ku80_1_shield |
| Subject ID: | SU001378 |
| Subject Type: | Cultured cells |
| Subject Species: | Toxoplasma gondii |
| Taxonomy ID: | 5811 |
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Collection:
| Collection ID: | CO001373 |
| Collection Summary: | Freshly egressing parasites were extracted through repeated syringe lysis (3x, 28G), purified from host cell material through filtration (3 μm pore size, Millipore/Merck) and pelleted by centrifugation (2,800g, 20 min, 4 °C). Pellets were washed with ice-cold PBS (3x) and metabolites were extracted in 250 μl chloroform:methanol:water (1:3:1). Samples were vortexed vigorously for 30 min at 4 °C and the insoluble material was pelleted through centrifugation (21,000g, 6 min, 4 °C). Samples were transferred to glass high-performance liquid chromatography (HPLC) vials and stored at −80 °C until analysis. |
| Collection Protocol Comments: | Sample Source/Type details: Human Foreskin Fibroblasts |
| Sample Type: | Fibroblasts |
