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MB Sample ID: SA094729
| Local Sample ID: | CI_3 |
| Subject ID: | SU001388 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
| Cell Primary Immortalized: | Islets isolated from pancreata of adult C57BL/J6 male mice |
| Cell Counts: | 100 islets |
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Collection:
| Collection ID: | CO001383 |
| Collection Summary: | Pancreatic islets were isolated from adult male C57BL/6 mice (Charles River Laboratories, Wilmington, MA). All procedures involving mice were approved by the University of North Carolina Institutional Animal and Use Committee. Mice were sacrificed by cervical dislocation and pancreas was infused in situwith collagenase P (1 mg/ml, Roche Diagnostics Corp., Indianapolis, IN) via the common bile duct. Pancreas was then removed and digested in the collagenase solution for 12 min at 37 °C. The digestate was washed and islets were purified by centrifugation in a gradient of Ficoll PM 400 (GE Healthcare, Uppsala, Sweden) (Szot et al., 2007).The isolated islets were cultivated overnight at 37 °C with 5% CO2 in RPMI 1640 medium (Mediatech, Manassas, VA) with 10% fetal bovine serum, 10 mM Hepes, 1 mM sodium pyruvate, 100 U/ml penicillin, and 100 μg/ml streptomycin (all from Sigma-Aldrich, St. Louis, MO). The same medium was used in experiments in which the islets were exposed to iAsIII (sodium arsenite; Sigma-Aldrich), MAsIII(methylarsine oxide) or DMAsIII (iododimethylarsine). |
| Sample Type: | Pancreas |
| Storage Conditions: | -80℃ |
