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MB Sample ID: SA097796
| Local Sample ID: | TLR7_WT_4 |
| Subject ID: | SU001418 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6/J |
| Age Or Age Range: | 8-12 |
| Gender: | Male |
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Collection:
| Collection ID: | CO001413 |
| Collection Summary: | Day 6 BMDMs were seeded at a density of 9x105 cell/well in 6 well dishes (Fisher 08-772-1B). On day 8, BMDM were mock-treated (media change) or co-treated with ligands as described below in 5% BMDM media for 48 h. Prior to collection, cell number was assessed by imaging as described below. After removal of culturing media, 1 mL of ice-cold PBS was added into each well and cells were scraped with cell lifters and spun down in glass tubes at 365g for 5 min at 4°C. |
| Sample Type: | Macrophages |
| Storage Conditions: | -80℃ |
