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MB Sample ID: SA120100

Local Sample ID:PA14_LB_3
Subject ID:SU001500
Subject Type:Bacteria
Subject Species:Staphylococcus epidermidis
Taxonomy ID:1282
Genotype Strain:ATCC 12600; ATCC 12228
Cell Biosource Or Supplier:ATCC
Cell Strain Details:ATCC 12600; ATCC 12228

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Collection:

Collection ID:CO001495
Collection Summary:Staphylococcus aureus (ATCC 12600) and Staphylococcus epidermidis (ATCC 12228) were cultured in four filter-sterilized complex media for metabolomics analysis: Brain Heart Infusion broth (BHI; Bacto); lysogeny broth Lennox (LB; Fisher Scientific); Mueller Hinton broth (MHB; Difco); and Tryptic Soy broth (TSB; Bacto). Each species was prepared in biological triplicate. Uninoculated media control blanks were prepared in six replicates for BHI, LB, and MHB, and in triplicate for TSB, following the same procedures as the bacterial samples and processed in parallel. Samples were transferred to capped GC vials and stored at -20 °C for approximately two weeks prior to GC×GC-TOFMS analysis.
Collection Protocol Filename:CLJenkins_Collection_Protocol_Metadata.txt
Sample Type:Bacterial cells
Collection Frequency:Once, at the completion of 24 hour aerobic incubation at 37 C with orbital shaking
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