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MB Sample ID: SA120702
| Local Sample ID: | WT-4 |
| Subject ID: | SU001503 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | Burkitt lymphoma cell line, wild type and two mutants, 4A and VP16 |
| Gender: | Not applicable |
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Collection:
| Collection ID: | CO001498 |
| Collection Summary: | To understand the cellular consequences of modulating the MYC–HCF-1 interaction, we engineered a system that allows us to express the 4A or VP16 HBM mutant MYC proteins as the sole form of MYC in a cell. We chose Ramos cells, a Burkitt lymphoma (BL)-derived line in which a t(8;14) translocation places one c-MYC allele under regulatory control of the immunoglobulin heavy chain enhancer. The untranslocated c-MYC allele is not expressed in these cells. Because sequences encoding the MYC HBM are contained within exon 3, we used CRISPR/Cas9-triggered homologous recombination of the translocated MYC allele to integrate an exon 3 switchable cassette for wild-type (WT) MYC, 4A, or VP16 HBM mutants, and confirmed appropriate integration by Southern blotting. Thus, we successfully generated a system for inducible, selective, and bidirectional modulation of the MYC−HCF-1 interaction in the context of an archetypal MYC-driven cancer cell line. |
| Sample Type: | Lymphoma cells |
| Storage Conditions: | -80℃ |
