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MB Sample ID: SA126250
| Local Sample ID: | Spike_00025_5 |
| Subject ID: | SU001572 |
| Subject Type: | Bacteria |
| Subject Species: | Fecal Bacteria |
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Collection:
| Collection ID: | CO001567 |
| Collection Summary: | One g of the human fecal sample was homogenized in anoxic phosphate-buffered saline (PBS, pH 7.0, Table 4) by vortexing with glass beads (c. 3 mm; Roth, Germany) to yield a 10% fecal suspension. The fecal suspension was further diluted to 1% in a Hungate tube containing anoxic PBS supplemented with 3.18 mM of sterile filtered Ti(III) nitrilotriacetate (Sigma-Aldrich, Germany) as reductant. Subsequently, fecal slurry aliquotes were centrifuged (14.000 x g, 4°C, 5 min) and the supernatant frozen until further processing. |
| Sample Type: | Feces |
