Return to study ST001547 main page
MB Sample ID: SA130683
| Local Sample ID: | CP_isoprenaline_1 |
| Subject ID: | SU001623 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO001616 |
| Collection Summary: | Cells (9.9x106) were differentiated in 10 cm glass dishes (Corning) coated with 10 mM fibronectin. After 24 hours in culture, cells were treated with 1 µM isoprenaline (or vehicle) for a further 24 hours. Cells were extracted as previously described (14). Briefly, cells were quenched and washed three times with 4 °C Dulbecco’s phosphate buffered saline (DPBS; Invitrogen), then scraped in 750 µL of ice-cold extraction solvent (chloroform: methanol: water = 1:3:1). After scraping, the samples were mixed thoroughly on a vortex mixer for 30 min at 1200 rpm (4 °C) and centrifuged at 20,000 g for 10 min (4 °C). Samples were then evaporated to dryness under a nitrogen stream and frozen at -80 °C until LC-MS analysis. |
| Sample Type: | Macrophages |
