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MB Sample ID: SA136794
| Local Sample ID: | 171009bBG_MediaBlk_002_1 |
| Subject ID: | SU001690 |
| Subject Type: | Bacteria |
| Subject Species: | Rothia mucilaginosa |
| Taxonomy ID: | 43675 |
| Genotype Strain: | RmFLR01 |
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Collection:
| Collection ID: | CO001683 |
| Collection Summary: | A quality control mixture of 29 unlabeled metabolite standards (Supporting information Table SI 1) was prepared to reach a final concentration of 1 mg/mL as stock solution. 50 µL aliquot from each stock solution was combined into a new tube and dried down. Then, the mixture was diluted to reach a final concentration of 50 µg/mL working solution. Rothia mucilaginosa strain RmFLR01 was isolated from a cystic fibrosis (CF) patient at the UC San Diego Adult CF Clinic [19, 32]. R. mucilaginosa cultures were grown in triplicates in artificial-sputum medium [33] spiked with 100 mM [U-13C6] d-glucose (Cambridge Isotope Laboratory, Tewksbury, MA, USA) under anaerobic and aerobic conditions (5% CO2) at 37°C and harvested at 4, 8, 12 and 24 h for isotope tracer analyses. |
| Sample Type: | Bacterial cells |
