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MB Sample ID: SA155500
Local Sample ID: | HFD-DEN_3D20.1L |
Subject ID: | SU001764 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl/6N |
Age Or Age Range: | 35 weeks |
Gender: | Male |
Animal Animal Supplier: | KU Leuven Animal Laboratory |
Animal Feed: | control diet (E15742-33 ssniff Spezialdiäten GmbH) or 60% high fat diet (S8655-E220 sniff Spezialdiäten GmbH) |
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Collection:
Collection ID: | CO001757 |
Collection Summary: | At endpoint, mice were sacrificed by injecting approximately 50 µl of a 60 mg/ml Nembutal solution (Vetoquinol). Tissues were immediately excised, washed in ice-cold saline, placed into pre-labelled bags and frozen using a liquid nitrogen-cooled biosqueezer. The bags were then placed in liquid nitrogen until all collections were finished and finally stored at -80°C until further processing. Where necessary tumors were rapidly separated from normal tissue prior to freezing with tumor and normal tissue being stored separately. |
Sample Type: | Liver |
Collection Method: | Surgical removal, tissue snap-freezing |
Storage Conditions: | -80℃ |