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MB Sample ID: SA163308
Local Sample ID: | NYSM-00483 |
Subject ID: | SU001822 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Long Evans |
Age Or Age Range: | From post-natal day 1 (PN1) to PN80 |
Weight Or Weight Range: | 5g-250g |
Gender: | Male and female |
Animal Animal Supplier: | Envigo |
Animal Housing: | Animal facility at NYU |
Animal Light Cycle: | 7am-7pm |
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Collection:
Collection ID: | CO001815 |
Collection Summary: | Rats trained in inhibitory avoidance at PN17, PN24, and PN80, and the untrained (naive) control rats were euthanized by decapitation (1 hour after IA training). Their brains were quickly removed and placed in ice-cold phosphate-buffered saline (1X). Whole hippocampal samples were dissected and immediately snap-frozen in isopentane on dry ice. All samples were stored at -80°C until processing. Frozen samples were shipped in dry ice to Metabolon Inc. (Morrisville, NC, USA) for metabolomic analysis using a proprietary methodology. Extraction of samples was performed using a MicroLab STAR automated liquid handling robot (Hamilton Robotics, Inc., Reno, NV, USA); 450 μL of methanol was added to 100 μo of sample to precipitate proteins. Four recovery standards (DL-2-fluorophenylglycine, tridecanoic acid, cholesterol-d6 and 4-chlorophenylalanine) were added to each sample to determine extraction efficiency. To remove proteins, dissociate small molecules bound to protein, and recover metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation (1300 g for 10 min at room temperature). The resultant supernatants were placed in a TurboVap (Zymark) to remove the organic solvent. Each sample extract was then divided into five equal aliquots, dried under nitrogen, and stored in vacuo prior to metabolomic profiling. |
Sample Type: | Brain |
Collection Method: | Brain dissection |
Collection Location: | New York University |
Storage Conditions: | -80℃ |