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MB Sample ID: SA163944
| Local Sample ID: | 18P |
| Subject ID: | SU001829 |
| Subject Type: | Bacteria |
| Subject Species: | Vibrio cholerae |
| Taxonomy ID: | 666 |
| Genotype Strain: | V. cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961- access:2018.06.26) |
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Collection:
| Collection ID: | CO001822 |
| Collection Summary: | V. cholerae wild-type, ΔvcdRP and ΔgltA each harboring an empty control plasmid (pCtrl) and ΔvcdRP with pVcdRP expression plasmids (pVcdRP and pVcdP) as well as ΔgltA harboring pVcdP were grown in LB medium to exponential and stationary phase, and were subsequently quenched in ice-cold methanol. The quenched samples were centrifuged at 3000 x g for 10 min at -9°C. 1ml aliquot of supernatant was stored for leakage analysis, while the rest of the pellets were flash frozen in liquid nitrogen. |
| Sample Type: | Bacterial cells |
