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MB Sample ID: SA174340
| Local Sample ID: | KG_2 |
| Subject ID: | SU001937 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | DSMZ |
| Cell Strain Details: | 8988T |
| Subject Comments: | pancreatic ductal adenocarcinoma |
| Cell Counts: | 1-2x10^6 |
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Collection:
| Collection ID: | CO001930 |
| Collection Summary: | Metabolites were initially extracted from samples by quickly aspirating the cell culture media and adding 1 mL of extraction buffer, consisting of 80% methanol (Fisher Scientific) and 500 nM metabolomics amino acid mix standard (Cambridge Isotope Laboratories). To effectively scale all harvested samples to equivalent volumes of extraction buffer, samples were fully dried down by Speedvac (Thermo Fisher, Waltham, MA) and reconstituted volumetrically by mixing the entire dried cell pellet sample with 1 mL of 80% methanol without QC standards in 2.0 mL screw cap vials containing ~100 µL of disruption beads (Research Products International, Mount Prospect, IL). Samples were scaled to a ratio of 1e6 cells to 1 mL of extraction solvent with all steps being carried out in a cold room. Each was homogenized for 10 cycles on a bead blaster homogenizer (Benchmark Scientific, Edison, NJ). Cycling consisted of a 30 sec homogenization time at 6 m/s followed by a 30 sec pause. Samples were subsequently spun at 21,000 x g for 3 min at 4°C. A set volume of each (450 µL) was transferred to a 1.5 mL tube and dried down by Speedvac concentration. Samples were reconstituted in 50 µL of Optima LC/MS grade water (Fisher Scientific, Waltham, MA). Samples were sonicated for 2 mins, then centrifuged at 21,000 x g for 3 min at 4°C. Twenty microliters were transferred to LC vials containing glass inserts for analysis. The remaining sample was placed in -80°C for long term storage. |
| Sample Type: | Cultured cells |
| Storage Conditions: | -80℃ |
