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MB Sample ID: SA174476
Local Sample ID: | 142 |
Subject ID: | SU001941 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Collection:
Collection ID: | CO001934 |
Collection Summary: | Banked blood (Saint Louis Children’s Hospital) and fresh blood [healthy donors] was washed and stored at 50% hematocrit at 4ºC up to 1 month past the clinical expiration date and collection date, respectively. Parasite strains provided by the Malaria Research and Reference Reagent Resource Center (MR4) as follows: 3D7 (MRA-102); K1 (MRA-159); D10 (MRA-201); and IPC-5202 (MRA-1240). Unless indicated, erythrocytes were cultured as previously described at 2% hematocrit in complete media (incomplete media supplemented with 27mM sodium bicarbonate, 11mM glucose, 5mM HEPES, 1mM sodium pyruvate, 0.37mM hypoxanthine, 0.01mM thymidine, 10ug/mL gentamicin, and 0.5% Albumax (Thermo-Fisher) under 5% O2/5% CO2/90% N2 atmosphere at 37ºC (71). |
Sample Type: | Blood (whole) |