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MB Sample ID: SA185680
| Local Sample ID: | OUHSC-27 |
| Subject ID: | SU002064 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
| Cell Biosource Or Supplier: | OVCAR4 cell line was obtained from Dr. Thomas Hamilton (Fox Chase Cancer Center, PA) and OVCAR8 cells were from National Cancer Institute (NCI). Kuramochi, TYKNU, OVKATE and OVSAHO cell lines were from the JCRB Cell Bank, Tokyo, Japan. SNU119, SNU251, ES-2, OVCAR3, CAOV3 and OV90 cells were from Seoul National University, Seoul, Korea, and COV362, OAW28 and COV318 cells were purchased from Sigma-Aldrich (St. Louis, MO). |
| Cell Passage Number: | 3 and 4 |
| Cell Counts: | 20 million cells |
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Collection:
| Collection ID: | CO002057 |
| Collection Summary: | Immortalized normal fallopian-tube-derived epithelial cells (FTE188) have been previously described and used here (1). High grade serous carcinoma cell line OVCAR3, OVCAR8, OVKATE, SNU119, SNU251, OVCAR4, OVSAHO, and Kuramochi cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium (Cellgro, Manassas, VA), COV362, COV318, OAW28 and CAOV3 cells were maintained in Dulbecco’s modified Eagle’s (DMEM) Medium (Cellgro, Manassas, VA). OV90 cells were maintained in MCDB105: M199 (1:1) Medium (Thermo Fisher Scientific, Waltham, MA) and TYKNU cells were maintained in Minimum Essential Medium (MEM) (Cellgro, Manassas, VA). ES-2 cells were maintained in McCoy’s 5A medium (Sigma-Aldrich, St. Louis, MO). All cells were maintained at 37°C in a 5% CO2 incubator. All media were supplemented with 10% FBS (Gemini Bio-Products, West Sacramento, CA), 50 U/mL penicillin, 50 μg/ml streptomycin (Cellgro, Manassas, VA). Cells were grown to 20 million cells and washed with cold PBS. Cells were collected by scraping them off the plates. |
| Sample Type: | Ovarian cancer cells |
| Storage Conditions: | -80℃ |
